Structure of the Cs1 Pilus of Enterotoxigenic Escherichia Coli
نویسندگان
چکیده
1 STRUCTURE OF THE CS1 PILUS OF ENTEROTOXIGENIC ESCHERICHIA COLI 1 REVEALS STRUCTURAL POLYMORPHISM 2 3 Running title: Structure of the ETEC CS1 pilus 4 5 Vitold E. Galkin*, Subramaniapillai Kolappan, Dixon Ng, ZuSheng Zong, Juliana Li, 6 Xiong Yu, Edward H. Egelman and Lisa Craig* 7 8 Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, 9 Charlottesville, Virginia, USA 22908 10 Molecular Biology and Biochemistry Department, Simon Fraser University, Burnaby, BC 11 Canada V5A 1S6 12 13 *Corresponding authors: [email protected], 434-243-6115 (phone), (fax): 434-924-5069; 14 [email protected], 778-782-7140 (phone) 778-782-5583 (fax). 15 16 These authors contributed equally to this work 17 18
منابع مشابه
The structure of the CS1 pilus of enterotoxigenic Escherichia coli reveals structural polymorphism.
Enterotoxigenic Escherichia coli (ETEC) is a bacterial pathogen that causes diarrhea in children and travelers in developing countries. ETEC adheres to host epithelial cells in the small intestine via a variety of different pili. The CS1 pilus is a prototype for a family of related pili, including the CFA/I pili, present on ETEC and other Gram-negative bacterial pathogens. These pili are assemb...
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Enterotoxigenic Escherichia coli associated with human diarrheal disease utilize any of a limited group of serologically distinguishable pili for attachment to intestinal cells. These include CS1 and CFA/I pili. We show here that chemical modification of arginyl residues in CS1 pili abolishes CS1-mediated agglutination of bovine erythrocytes, which serves as a model system for attachment. Alani...
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Background & Objective: Enterotoxigenic Escherichia coli (ETEC) is a major causative agent of diarrhea. Enterotoxins and the colonization factors (CFs) are major virulence factors in ETEC infections. The bacterium binds to the intestinal epithelial cell surface through colonization factors and produces enterotoxins that cause excessive fluid and electrolyte secretion in the lumen of the intesti...
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CS1 pili serve as the prototype of a class of filamentous appendages found on the surface of strains of enterotoxigenic Escherichia coli. The four genes needed to synthesize functional CS1 pili in E. coli K12 are: cooA, which encodes the major pilin protein; cooD, which encodes a minor pilin protein found at the tip of the structure; cooC, which encodes a protein found in the outer membrane of ...
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CooD, the minor subunit of CS1 pili of enterotoxigenic Escherichia coli, is essential for the assembly of stable, functional pili. We previously proposed that CooD is a rate-limiting initiator of CS1 pilus assembly and predicted that the level of CooD expression should therefore determine the number of CS1 pili assembled on the cell surface. In this study, we confirm that CooD is required for t...
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